Multi-Objective Multiplex PCR Design Version 2.2 (July 28, 2006) Help   Contact   Disclaimer

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PRIMER SELECTION CRITERIA Min Max Length bp %GC % Tm C Product bp Product Diff bp New! 3'/5' Dist bp Base Repeat 3'Tail ΔG kCal/mol Max Tm Diff C Align Score Match Mismatch Gap Penalty BLAT Filter? bp ProdSz Primer Pairs (500 max)

SEQUENCES Assay name: (optional) File: Manual Entry: Test Samples Please cite: Rachlin J., Ding CM., Cantor C., and Kasif S.(2005). muPlex: multi-objective multiplex PCR assay design. Nucleic Acids Research. 33(Web Server Issue):W544-7. --- Paste FASTA-formatted sequences below. --- Data Format: Sequence Only Sequence Brackets ...[...]... SNP Brackets [C/G] SNP IUPAC Ambiguity Code Mask Regions: NNNN or XXXX Lowercase atgc Target Plex: Algorithm: 1 solution (fastest) Limited tradeoffs Deep search (slowest)